Journal: The Journal of Biological Chemistry

Article Title: Blom7α Is a Novel Heterogeneous Nuclear Ribonucleoprotein K Homology Domain Protein Involved in Pre-mRNA Splicing That Interacts with SNEV Prp19-Pso4 *

doi: 10.1074/jbc.M109.036632

Figure Lengend Snippet: Putative interaction partners of SNEV Prp19-Pso4 as identified by yeast two-hybrid screening against a human aorta cDNA library

Article Snippet: As cDNA library for identification of novel putative interactors, the pACT2 plasmid-based human aorta cDNA library was used (Clontech).

Techniques: Two Hybrid Screening

Journal: The Journal of Biological Chemistry

Article Title: Blom7α Is a Novel Heterogeneous Nuclear Ribonucleoprotein K Homology Domain Protein Involved in Pre-mRNA Splicing That Interacts with SNEV Prp19-Pso4 *

doi: 10.1074/jbc.M109.036632

Figure Lengend Snippet: Blom7α-specific C terminus and self-interaction domain of SNEV Prp19-Pso4 are necessary for interaction. A , C-terminal half of amino acid 347–614 were isolated from the human aorta cDNA library. B , Blom7α deletion mutants identify the α-specific C terminus as necessary for the interaction, whereas on the side of SNEV Prp19-Pso4 . C , the self-interaction domain is necessary as determined by directed yeast two-hybrid analyses under high stringent conditions. U-box , UFD2-like motif with E3 ligase activity. SID , self-interaction domain; LCR , low complexity region; CC , coiled-coil domain, GL2 , globular domain; LCR1 , low complexity region APG-rich; KH1, -2 , KH domain (RNA binding domain); LCR2 , low complexity region PS-rich; α, α-specific C terminus; β, β-specific C terminus; γ, γ-specific C terminus.

Article Snippet: As cDNA library for identification of novel putative interactors, the pACT2 plasmid-based human aorta cDNA library was used (Clontech).

Techniques: Isolation, cDNA Library Assay, Activity Assay, RNA Binding Assay